18.3: Donor testing 18.3.1: HPA typing Donors whose products may be used for fetal/neonatal transfusions should be HPA typed twice using samples collected on diff…
18.2: Methods 18.2.1: HPA typing methods HPA types should be determined using antibody-based and/or DNA/PCR-based techniques that have been validated in the laboratory.…
18.1: Reagent manufacture/reference preparations 18.1.1: HPA typing reagents There are several human platelet antigen (HPA) genotyping and phenotyping techniques. The <…
16.3: Terminology and nomenclature All HLA assignments, irrespective of the method, must comply with the latest report of the WHO Nomenclature Committee for Factors of the HLA System.
15.2: Clinical applications of blood group molecular typing Various clinical applications of blood group molecular typing are listed below: Fetal typing:1
14.1: Safety precautions All human cells should be treated as potentially infectious. Materials should be handled and discarded according to in-house documented procedures for potentiall…
13.5: Fetomaternal haemorrhage estimation by flow cytometry 13.5.1 Indications for testing For any D negative pregnant mother where the screening test indicates a fetomaternal…
13.6: Post-examination All patient records and test results should be maintained according to the requirements of the Caldicott Report (1997)8 and Data Protection…
13.4: Antibody quantification and titration Antibody quantification and titration is performed in RCI laboratories on patients’ samples, to support the prediction and management of HDFN …
13.3: Pre-transfusion testing 13.3.1: Resolution of anomalous grouping ABO grouping is the most important pre-transfusion serological test performed. Fully automated ABO and D…
13.2: Sample acceptance and labelling Visual inspection to determine the suitability for testing should consider the following in relation to the equipment, methods and samples used:…
13.1: Scope These specifications provide guidance on the tests required for investigations performed on patient samples in red cell immunohaematology (RCI) laboratories in UK Blood Trans…
5.12: Donor compensation The Blood Transfusion Services should have established procedures to ensure that any claim by a donor for compensation for any injury or loss allege…
5.11: Adverse events All adverse events must be documented and reported according to standard protocols. All bag/harness defects (e.g. pinhole leaks) must be recorded and all de…
5.9: Information to be provided to the donor post-donation The donor must be provided with information on care of the venepuncture site and requested to report any illness o…
5.8: Component donation by apheresis Guidance for collection procedures is identical to that for normal whole blood donations except for the points listed below.
5.7: Whole blood donation If necessary, the donor should be asked to open and close his/her hand slowly every 10–12 seconds to encourage a free flow of blood.
5.6: Performance of the venepuncture Venepuncture should only be undertaken by authorised and trained personnel. Items used for venepuncture must be st…
5.5: Preparation of the blood pack 5.5.1: Whole blood pack The blood collection set must be in date and inspected for any defects. These are sometimes obscured by…
5.4: Preparation of the venepuncture site Blood must be drawn from a suitable vein in the antecubital fossa in an area that is free of skin lesions. The veins can be made mo…
5.3: Haemoglobin screening A validated haemoglobin screen should be applied to all donors prior to donation. The objective is to ensure that prior to …
5.2: Information to be obtained from donors by Blood Establishments at every donation 5.2.1: Donor identification Donors must positively identify themselves by volunteering th…
5.1: Information to be provided to prospective donors of blood or blood components The following information must be provided to all donors: Accurate educational material…
4.8: Protection and preservation of donation quality All blood and components must be transported, tested and stored in accordance with the specificat…
4.6: Records It is strongly recommended that all records pertaining to donor and donation identity be entered and maintained in an electronic format which can be accessed re…